Tune in to hear all about Pixelgen Technologies - creators of a novel technology for doing single-cell spatial proteomics.

We chat about how this fascinating technology works, what you can do with the data and why they chose to develop their analysis suite as an open-source nf-core pipeline.

Links:

• Software:
  • nf-core/pixelator pipeline
  • Pixelator Python package
• Science:
  • Nature Methods publication: “Molecular pixelation: spatial proteomics of single cells by sequencing”
• Presentations:
  • Nextflow Summit 2022 talk: “Pixelgen Technologies ❤︎ Nextflow”
  • Nextflow Summit 2024 Boston talk: “Nextflow allows Pixelgen innovation engine to reach the market quicker”
  • Nextflow Summit 2024 Barcelona
• Pixelgen:
  • Pixelgen Technologies homepage
  • @PixelgenTech on twitter / X and LinkedIn
• BioLizard:
  • BioLizard homepage
  • @BioLizard_nv on twitter / X and LinkedIn

Video chapters (links to YouTube):

• 00:00 - Start
• 00:09 - Welcome
• 00:50 - Introductions
• 01:41 - Intro to Pixelgen Technologies
• 03:08 - Lab methods
• 06:15 - Nature Methods publication
• 09:35 - Data visualisation
• 12:29 - Working with single cell data
• 15:06 - Cell interactions
• 16:05 - Why Pixelgen chose open source
• 20:10 - Pixelator Python package
• 21:40 - Building within nf-core
• 23:01 - Florian - Biolizard
• 25:19 - Pipeline overview
• 26:50 - Using nf-core from a commercial setting
• 28:53 - Coming out of stealth
• 32:24 - Extending Pixelator
• 34:58 - Dealing with customers
• 39:38 - Looking to the future
• 41:32 - Interactive analysis
• 43:12 - Advice for others
• 45:16 - Pixelgen at the Nextflow Summit